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- /*
- * nvbio
- * Copyright (c) 2011-2014, NVIDIA CORPORATION. All rights reserved.
- *
- * Redistribution and use in source and binary forms, with or without
- * modification, are permitted provided that the following conditions are met:
- * * Redistributions of source code must retain the above copyright
- * notice, this list of conditions and the following disclaimer.
- * * Redistributions in binary form must reproduce the above copyright
- * notice, this list of conditions and the following disclaimer in the
- * documentation and/or other materials provided with the distribution.
- * * Neither the name of the NVIDIA CORPORATION nor the
- * names of its contributors may be used to endorse or promote products
- * derived from this software without specific prior written permission.
- *
- * THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND
- * ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED
- * WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE
- * DISCLAIMED. IN NO EVENT SHALL NVIDIA CORPORATION BE LIABLE FOR ANY
- * DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES
- * (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES;
- * LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND
- * ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT
- * (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS
- * SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.
- */
- // seeding.cu
- //
- #include <stdio.h>
- #include <stdlib.h>
- #include <nvbio/basic/console.h>
- #include <nvbio/basic/vector.h>
- #include <nvbio/basic/shared_pointer.h>
- #include <nvbio/strings/string_set.h>
- #include <nvbio/strings/infix.h>
- #include <nvbio/strings/seeds.h>
- #include <nvbio/io/sequence/sequence.h>
- using namespace nvbio;
- // extract a set of uniformly spaced seeds from a string-set and return it as an InfixSet
- //
- template <typename system_tag, typename string_set_type>
- InfixSet<string_set_type, const string_set_infix_coord_type*>
- extract_seeds(
- const string_set_type string_set, // the input string-set
- const uint32 seed_len, // the seeds length
- const uint32 seed_interval, // the spacing between seeds
- nvbio::vector<system_tag,string_set_infix_coord_type>& seed_coords) // the output vector of seed coordinates
- {
- // enumerate all seeds
- const uint32 n_seeds = enumerate_string_set_seeds(
- string_set,
- uniform_seeds_functor<>( seed_len, seed_interval ),
- seed_coords );
- // and build the output infix-set
- return InfixSet<string_set_type, const string_set_infix_coord_type*>(
- n_seeds,
- string_set,
- nvbio::plain_view( seed_coords ) );
- }
- // main test entry point
- //
- int main(int argc, char* argv[])
- {
- //
- // perform some basic option parsing
- //
- uint32 n_bps = 10000000;
- const char* reads = "./data/SRR493095_1.fastq.gz";
- for (int i = 0; i < argc; ++i)
- {
- if (strcmp( argv[i], "-bps" ) == 0)
- n_bps = uint32( atoi( argv[++i] ) )*1000u;
- else if (strcmp( argv[i], "-reads" ) == 0)
- reads = argv[++i];
- }
- // start our program
- log_info(stderr, "seeding... started\n");
- // open a read file
- log_info(stderr, " loading reads... started\n");
- SharedPointer<io::SequenceDataStream> read_data_file(
- io::open_sequence_file(
- reads,
- io::Phred33,
- uint32(-1),
- uint32(-1) ) );
- // check whether the file opened correctly
- if (read_data_file == NULL || read_data_file->is_ok() == false)
- {
- log_error(stderr, " failed opening file \"%s\"\n", reads);
- return 1u;
- }
- const uint32 batch_size = uint32(-1);
- const uint32 batch_bps = n_bps;
- // load a batch of reads
- io::SequenceDataHost h_read_data;
- io::next( DNA_N, &h_read_data, read_data_file.get(), batch_size, batch_bps );
- // copy it to the device
- const io::SequenceDataDevice d_read_data( h_read_data );
- log_info(stderr, " loading reads... done\n");
- log_info(stderr, " %u reads\n", d_read_data.size());
- // prepare some typedefs for the involved string-sets and infixes
- typedef io::SequenceDataAccess<DNA_N> read_access_type;
- typedef read_access_type::sequence_string_set_type string_set_type; // the read string-set
- typedef string_set_infix_coord_type infix_coord_type; // the infix coordinate type, for string-sets
- typedef nvbio::vector<device_tag,infix_coord_type> infix_vector_type; // the device vector type for infix coordinates
- typedef InfixSet<string_set_type, const string_set_infix_coord_type*> seed_set_type; // the infix-set type for representing seeds
- // build a read accessor
- const read_access_type d_read_access( d_read_data );
- // fetch the actual read string-set
- const string_set_type d_read_string_set = d_read_access.sequence_string_set();
- // prepare enough storage for the seed coordinates
- infix_vector_type d_seed_coords;
- // extract the seeds and get the corresponding string-set representation
- const seed_set_type d_seed_set = extract_seeds(
- d_read_string_set,
- 20u,
- 10u,
- d_seed_coords );
- // output some stats
- log_info(stderr, "seeding... done\n");
- log_info(stderr, " %u seeds\n", d_seed_set.size());
- return 0;
- }
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